Cell-Free DNA Blood Collection Tube

●  Features

cfDNA preservation：unique formular of anticoagulation and preservation reagents, that can stabilize cfDNA for 7 days or more at room temperature

Blood cess fixation：prevent  releasing of cell genomic DNA so as not to contaminate targeting cfDNA

Double-coded label design：ensure the identification of each individual tube reduce sample processing errors;

●  Product Performance

Fig. After 5 days, the nucleated cells preserved in the BCT of S company severely ruptured, releasing a large amount of DNA into the plasma. Ardent BCT can efficiently stabilize nucleated cells and prevent the release of genomic DNA into plasma

Fig. After 5 days, the expression of Rnase P gene in the BCT of S company decreased. Ardent BCT can effectively inhibit nuclease activity and protect free DNA from degradation

Super NGS Library Yield and Quality

The blood samples were collected into Ardent BCT and conventional EDTA BCT. After being placed at room temperature for 5 days, the fragment size and peak pattern of free DNA were detected by a bioanalyzer. It can be seen that conventional EDTA BCT cannot prevent cfDNA degradation

Sequencing Fig. The read length of the NGS library of conventional EDTA BCT is shorter than that of Ardent BCT. Ardent BCT can better preserve free DNA and obtain high-quality libraries

Fig. Ardent BCT can store a higher yield of NGS library than S company BCT

Fig. Through sequencing, it was found that the samples collected by Ardent BCT could obtain a higher number of effective reads and more uniform GC content than those of S company